Technical Service
Service Introduction
Development of monoclonal cell lines
Vector construction, and the exogenous gene was introduced into CHO/HEK293 cells by transfection, and the resistance gene was screened. The single cell printing system and monoclonal imaging system were combined to screen the monoclonal cell line with stable and high-throughput expression of the target protein.
Inquiry
Service Advantages
1、Mature HEK293/CHO cell expression platform
2、VIPS, Cell Metric high-throughput cell screening system
3、Possess experience in developing cell lines for a variety of cytokines, fusion proteins, and antibodies, as well as a complete cell line development and upstream process development process
Service Content
| Serial number | Service Content | Deliverables |
| 01 | Cell pool development and evaluation | Stable high-yield engineered cell lines |
| 02 | Cell line development and stability assessment | Monoclonal report |
| 03 | Cell bank establishment | Research cell bank for engineered cell lines |
Project Process
- DNA synthesis and plasmid construction
- Cell pool screening
- Clone screening
- TOP3-6 screening and RCB establishment
- TOP1 screening
Analytical methods
HPLC, SDS-PAGE, Elisa, WB, etc.
